Journal: Journal of visualized experiments : JoVE

Article Title: Traction microscopy integrated with microfluidics for chemotactic collective migration

doi: 10.3791/60415

Figure Lengend Snippet: Table of Materials/Equipment

Article Snippet: 1 M HEPES buffer solution Gibco 15630-056 Ultraviolet (UV) lamp UVP LLC 95-0248-02 365 nm wavelength Collagen typeI, Rat tail Corning 354236 Dulbecco’s Phosphate Buffered Saline (PBS) Biowest L0615-500 w/o Magnesium, Calcium Pluronic F-127 Sigma-Aldrich P2443-250G Madin-Darby Canine Kidney (MDCK) cell type II Dulbecco’s Modified Eagle’s Medium (DMEM) Welgene LM 001-11 Fetal bovine serum (FBS) Gibco 26140-179 Antibiotic-Antimycotic Gibco 15240-062 0.25% trypsin-EDTA (1X) Gibco 25200-056 Oxygen plasma system Femto Science CUTE-MPR Custom glass holder Han-Gug Mechatronics custom-made Steril hypodermic needle 18 G KOVAX Trim the tip of the needle and bend it 90 degrees for connecting in/out ports with volume line Syringes KOVAX 1, 3, 5, 10, or 50 cc for using inlet reservoir or outlet syringe pump 75 cm minimum volume line (for pediatric) Hyupsung HS-MV-75 CAUTION: do not use if previously opened. do not resterlize or resuse 30 cm minimum volume line (for pediatric) Hyupsung HS-MV-30 CAUTION: do not use if previously opened. do not resterlize or resuse 3-way stopcock Hyupsung HS-T-61N CAUTION: do not use if previously opened. do not resterlize or resuse Syringe pump Chemyx Inc. model fusion 720 withdraw fluid Hepatocyte Growth Factor (HGF) Sigma-Aldrich H1404-5UG recombinant, human Rhodamine B isothiocyanate–dextran Sigma-Aldrich R9379-100MG 70 kDa, used to estimate spatiotemporal distribution of HGF in the microfluidic channel JuLI stage live cell imaging system NanoEnTek Automated X-Y-Z stage and fluorsent imaging Incubator-compatible (37 °C and 5% CO2) Open in a separate window Table of Materials/Equipment

Techniques: Cell Culture, Modification, Recombinant, Live Cell Imaging, Imaging